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Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal <t>4PL</t> method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)
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Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal 4PL method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)

Journal: Thoracic Cancer

Article Title: Harmonization across programmed death ligand 1 ( PD‐L1) assays for lung cancer by immunohistochemistry using noncontact alternating current electric field mixing

doi: 10.1111/1759-7714.13893

Figure Lengend Snippet: Comparison of the percentage of tumor cell staining for each programmed death ligand 1 (PD‐L1) assay (the 22C3, 28–8, SP263, and SP142 assays), by case, with/without alternating current (AC) mixing. (a) Conventional immunohistochemistry (IHC) ( ) 22C3, ( ) 28‐8, ( ) SP263, ( ) SP142. (b) AC mixing IHC ( ) 22C3, ( ) 28‐8 AC, ( ) SP263 AC, ( ) SP142 AC. The standard IHC is the 22C3 phamDx IHC on Autostainer Link 48 platform in both figures. Best fit colored curves enable comparison of score ranges between the four assays. When assessing PD‐L1 TPS in the cases of 22C3 PD‐L1 >1%‐positive patients using AC mixing, the nonlinear best fit curve calculated using the sigmoidal 4PL method showed one curve for all four PD‐L1 antibodies (extra sum‐of squares F test, p = 0.2552)

Article Snippet: Best fit curves data were fitted in GraphPad using the Sigmoidal 4PL option.

Techniques: Comparison, Staining, Immunohistochemistry